![Western Blot: Radixin Antibody (1D9) [H00005962-M06] - Analysis of RDX expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Radixin-Antibody-1D9-Western-Blot-H00005962-M06-img0004.jpg "Western Blot: Radixin Antibody (1D9) [H00005962-M06] - Analysis of RDX expression in HeLa (Cat # L013V1).")
| Reactivity | Hu, Mu, Rt, ChSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 1D9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | RDX (AAH47109, 1 a.a. ~ 583 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTVGLREVWFFGLQYVDSKGYSTWLKLNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRLFFLQVKEAILNDEIYCPPETAVLLASYAVQAKYGDYNKEIHKPGYLANDRLLPQRVLEQHKLTKEQWEERIQNWHEEHRGMLREDSMMEYLKIAQDLEMYGVNYFEIKNKKGTELWLGVDALGLNIYEHDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERAQLENEKEKREIAEKEKERIEREKEELMERLKQIEEQTIKAQKELEEQTRKALELDQERKRAKEEAERLEKERRAAEEAKSAIAKQAADQMKNQEQLAAELAEFTAKIALLEEAKKKKEEEATEWQHKAFAAQEDLEKTKEELKTVMSAPPPPPPPPVIPPTENEHDEHDENNAEASAELSNEGVMNHRSEEERVTETQKNERVKKQLQALSSELAQARDETKKTQNDVLHAENVKAGRDKYKTLRQIRQGNTKQRIDEFEAM |
| Specificity | RDX - radixin |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RDX |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Radixin Antibody (H00005962-M06)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Analysis of monoclonal antibody to RDX on HeLa cell. Antibody concentration 30 ug/ml](http://images.novusbio.com/fullsize/Radixin-Antibody-1D9-Immunocytochemistry-Immunofluorescence-H00005962-M06-img0003.jpg "Immunocytochemistry/Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Analysis of monoclonal antibody to RDX on HeLa cell. Antibody concentration 30 ug/ml")
![Immunohistochemistry: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. Merged image of radixin and phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00161/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Radixin-Antibody-1D9-Immunohistochemistry-H00005962-M06-img0005.jpg "Immunohistochemistry: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. Merged image of radixin and phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00161/abstract) licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. (A) Merged image of radixin & phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. (B) pERM immunoreactivity in the control RMS. (C) Merged image of radixin & phosphorylated ezrin/radixin/moesin (pERM) immunoreactivity in a coronal section in the DX52-1 treated RMS. (D) pERMimmunoreactivity in the DX52-1 treated RMS. Radixin/pERM immunopositive circular structures in B & D are likely residual ependymal cells originating from the wall of the collapsed olfactory ventricle (Peretto et al., 1997). Ependymal cells are known to express high levels of ezrin which explains sustained pERM expression in this area in both control & DX52-1 treated RMS. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24065889), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005962-m06_mouse-monoclonal-radixin-antibody-1d9-31020241548510.jpg "Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. (A) Merged image of radixin & phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. (B) pERM immunoreactivity in the control RMS. (C) Merged image of radixin & phosphorylated ezrin/radixin/moesin (pERM) immunoreactivity in a coronal section in the DX52-1 treated RMS. (D) pERMimmunoreactivity in the DX52-1 treated RMS. Radixin/pERM immunopositive circular structures in B & D are likely residual ependymal cells originating from the wall of the collapsed olfactory ventricle (Peretto et al., 1997). Ependymal cells are known to express high levels of ezrin which explains sustained pERM expression in this area in both control & DX52-1 treated RMS. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24065889), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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