![Western Blot: ZEB1 Antibody [NBP1-68930] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: ZEB1.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-68930-img0005.jpg "Western Blot: ZEB1 Antibody [NBP1-68930] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: ZEB1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Description | The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
| Immunogen | Synthetic peptides corresponding to ZEB1 (zinc finger E-box binding homeobox 1) The peptide sequence was selected from the N terminal of ZEB1. Peptide sequence NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT. The peptide sequence for this immunogen was taken from within the described region. |
| Marker | Mesenchymal Cells Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ZEB1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 124 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 2% Sucrose |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Samantha Garcia |
WB | Other | 11/05/2013 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for ZEB1 Antibody (NBP1-68930)Find related products by research area.
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi... Read full blog post. |
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![Immunohistochemistry: ZEB1 Antibody [NBP1-68930] - Human Capan1 cells (Pancreatic cancer cell line) Primary Antibody Dilution: 1 : 300 Secondary Antibody: Anti-rabbit-AlexaFluor-488 Secondary Antibody Dilution: 1 : 200Color/Signal.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-NBP1-68930-img0004.jpg "Immunohistochemistry: ZEB1 Antibody [NBP1-68930] - Human Capan1 cells (Pancreatic cancer cell line) Primary Antibody Dilution: 1 : 300 Secondary Antibody: Anti-rabbit-AlexaFluor-488 Secondary Antibody Dilution: 1 : 200Color/Signal.")
![Western Blot: ZEB1 Antibody [NBP1-68930] - Lane 1: Control, Lane 2: ZEB1 morphants. Upper panel: ZEB1 blot; Bottom panel: tubulin blot](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-68930-img0003.jpg "Western Blot: ZEB1 Antibody [NBP1-68930] - Lane 1: Control, Lane 2: ZEB1 morphants. Upper panel: ZEB1 blot; Bottom panel: tubulin blot")
![Western Blot: ZEB1 Antibody [NBP1-68930] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-68930-img0002.jpg "Western Blot: ZEB1 Antibody [NBP1-68930] - 293T cells lysate, concentration 0.2-1 ug/ml.")
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