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GEMIN2 Antibody (OTI4C11)

Images

 
Western Blot: SIP1 Antibody (4C11) [NBP2-45831] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GEMIN2 .
Immunohistochemistry: GEMIN2 Antibody (4C11) [NBP2-45831] - SIP1 Antibody (4C11) [NBP2-45831] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: GEMIN2 Antibody (4C11) [NBP2-45831] - SIP1 Antibody (4C11) [NBP2-45831] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: GEMIN2 Antibody (4C11) [NBP2-45831] - SIP1 Antibody (4C11) [NBP2-45831] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GEMIN2 Antibody (OTI4C11) Summary

Immunogen
Full length human recombinant protein of human GEMIN2(NP_003607) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GEMIN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GEMIN2 Antibody (OTI4C11)

  • Component of gems 2
  • Gemin-2
  • GEMIN2survival of motor neuron protein-interacting protein 1
  • SIP1-delta
  • SMN interacting protein 1-delta
  • SMN-interacting protein 1
  • survival of motor neuron protein interacting protein 1

Background

SIP1 encodes one of the proteins found in the SMN complex, which consists of the suvival of motor neuron protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GEMIN2