Reactivity | HuSpecies Glossary |
Applications | Simple Western, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
Clone | 2F3.2 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | CoraFluor 1 |
Description | CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays. |
Immunogen | Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (Uniprot: P43403) |
Localization | Cell Surface and Cytoplasmic |
Marker | Chronic Lymphocytic Leukemia Marker |
Specificity | ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive Purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis. |
Isotype | IgG2a Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | ZAP70 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Optimal dilution of this antibody should be experimentally determined. |
Storage | Store at 4C in the dark. Do not freeze. |
Buffer | PBS |
Preservative | No Preservative |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ZAP70 Antibody (NBP2-47774CL1)Find related products by research area.
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Gene Symbol | ZAP70 |