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YY1 Antibody (854847)

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Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. Gels were loaded with 30 µg of U937 and 40 µg of C2C12 cytoplasmic (Cyto) extracts and 15 µg of U937 and 20 µg of C2C12 nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse YY1 Monoclonal Antibody (Catalog # MAB3784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YY1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. Gels were loaded with 30 µg of U937 and 40 µg of C2C12 cytoplasmic (Cyto) extracts and 15 µg of U937 and 20 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
854847
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3784-SP
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YY1 Antibody (854847) Summary

Immunogen
E. coli-derived recombinant human YY1
His75-Gln275
Accession # P25490
Specificity
Detects human YY1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Rex-1/ZFP42 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
YY1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publication using
MAB3784 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for YY1 Antibody (854847)

  • Delta transcription factor
  • DELTA
  • INO80SYY-1
  • NF-E1
  • NF-E1INO80 complex subunit S
  • transcriptional repressor protein YY1
  • UCRBP
  • Yin and Yang 1 protein
  • Yin and yang 1
  • YIN-YANG-1
  • YY1 transcription factor
  • YY1

Background

YY1 (Yin Yang 1) is a multifunctional transcription factor belonging to the GLI-Kruppel class of zing finger proteins. YY1 is essential for embryogenesis, apoptosis, differentiation, cell growth and proliferation. YY1 binds to the DNA consensus site CGCCATNTT. It can function as an initiator of transcription as well as activating or repressing transcription through protein complexes (e.g. histone deacetylases and histone acetyltransferases).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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YY1 Antibody
(854847)
MAB3784
YY1 Antibody (854847)
Species: Hu, Mu
Applications: WB

Publications for YY1 Antibody (MAB3784)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol YY1
Uniprot