Name: YM1/Chitinase 3-like 3 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF2446
Rating: 5 (1 reviews)

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Immunohistochemistry-Frozen YM1/Chitinase 3-like 3 AF2446

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reviewed by:
Branislav Krljanac

IHC-Fr

Mouse

07/16/2017

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Summary

Application	Immunohistochemistry-Frozen
Sample Tested	Nippostrongylus brasiliensis infected mouse lung (day 14 post infection)
Species	Mouse
Lot	UNU0115051

Comments

Nippostrongylus brasiliensis infected mouse lung (day 14 post infection)
Frozen section (acetone fixed)
Blocking in 2,5% donkey serum (30min)
Goat-Ym-1 (AF2446) 1/100 in PBS+2,5% BSA (1h)
Donkey-anti-Goat-A488 1/150 in 2,5% donkey serum (1h)
Mounting media containing DAPI.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	WB, Simple Western
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF2446

Catalog# & Formulation	Size	Price
Carrier Free AF2446-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2446

YM1/Chitinase 3-like 3 Antibody [Unconjugated]

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YM1/Chitinase 3-like 3 Antibody [Unconjugated] Summary

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Alternate Names for YM1/Chitinase 3-like 3 Antibody [Unconjugated]

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Publications for YM1/Chitinase 3-like 3 Antibody (AF2446)(25)

Review for YM1/Chitinase 3-like 3 Antibody (AF2446) (1) 51

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Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like 3 Tyr22-Tyr398 Accession # O35744
Specificity	Detects mouse YM1/Chitinase 3-like 3 in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	CHI3L2
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF2446	Applications	Species
Hamley, M;Leyk, S;Casar, C;Liebold, I;Jawazneh, AA;Lanzloth, C;Böttcher, M;Haas, H;Richardt, U;Rothlin, CV;Jacobs, T;Huber, S;Adlung, L;Pelczar, P;Henao-Mejia, J;Bosurgi, L; Nmes1 is a novel regulator of mucosal response influencing intestinal healing potential European journal of immunology 2023-11-16 [PMID: 37971166] (Flow Cytometry, Transgenic Mouse, Mouse)	Flow Cytometry	Transgenic Mouse, Mouse
F Yu, T Bai Sevoflurane activates the IL-6/HO-1 pathway to promote macrophage M2 polarization and prostate cancer lung metastasis International immunopharmacology, 2022-10-30;113(0):109380. 2022-10-30 [PMID: 36330914] (Western Blot, Human)	Western Blot	Human
Georgios Theocharidis, Sahar Rahmani, Sangmin Lee, Zhuqing Li, Antonio Lobao, Konstantinos Kounas et al. Murine macrophages or their secretome delivered in alginate dressings enhance impaired wound healing in diabetic mice Biomaterials 2022-09-01 [PMID: 35934520]
Fleming Martinez A, DOppler H, Bastea L et al. Ym1+ macrophages orchestrate fibrosis, lesion growth and progression during development of murine pancreatic cancer iScience 4/1/2022 [PMID: 35602933]
Jianrui Song, Ryan A. Frieler, Thomas M. Vigil, Jun Ma, Frank Brombacher, Sascha N. Goonewardena et al. Inactivation of Interleukin‐4 Receptor alpha Signaling in Myeloid Cells Protects Mice From Angiotensin II/High Salt–Induced Cardiovascular Dysfunction Through Suppression of Fibrotic Remodeling Journal of the American Heart Association 2021-07-06 [PMID: 34132103]
Y Yuan, Y Ren, M Dijk, GI Geertsema-, J Atema-Smit, HJ Busscher, HC van der Me Phagocytosis and macrophage polarization on bacterially contaminated dental implant materials and effects on tissue integration European cells & materials, 2021-03-30;41(0):421-430. 2021-03-30 [PMID: 33782938] (ICC, Mouse)	ICC	Mouse
Jessica M. Snyder, Guo Zhong, Cathryn Hogarth, Weize Huang, Traci Topping, Jeffrey LaFrance et al. Knockout of Cyp26a1 and Cyp26b1 during postnatal life causes reduced lifespan, dermatitis, splenomegaly, and systemic inflammation in mice The FASEB Journal 2020-12-01 [PMID: 33105029]
D Cerezo-Wal, M Contreras-, K Troulé, X Catena, C Mucientes, TG Calvo, E Cañón, C Tejedo, PC Pennacchi, S Hogan, P Kölblinger, H Tejero, AX Chen, N Ibarz, O Graña-Cast, L Martinez, J Muñoz, P Ortiz-Rome, JL Rodriguez-, G Gómez-Lópe, F Al-Shahrou, R Rabadán, MP Levesque, D Olmeda, MS Soengas Midkine rewires the melanoma microenvironment toward a tolerogenic and immune-resistant state Nat Med, 2020-10-19;0(0):. 2020-10-19 [PMID: 33077955] (IHC, Mouse)	IHC	Mouse
AL Chenery, R Alhallaf, Z Agha, J Ajendra, JE Parkinson, MM Cooper, BHK Chan, RM Eichenberg, LA Dent, AAB Robertson, A Kupz, D Brough, A Loukas, TE Sutherland, JE Allen, PR Giacomin Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung J. Immunol., 2019-10-04;203(10):2724-2734. 2019-10-04 [PMID: 31586037] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
M Ryzhikov, A Ehlers, D Steinberg, W Xie, E Oberlander, S Brown, PE Gilmore, RR Townsend, WS Lane, T Dolinay, K Nakahira, AMK Choi, JA Haspel Diurnal Rhythms Spatially and Temporally Organize Autophagy Cell Rep, 2019-02-12;26(7):1880-1892.e6. 2/12/2019 [PMID: 30759397]
LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism Mucosal Immunol, 2018-10-18;0(0):. 2018-10-18 [PMID: 30337651] (IHC, Mouse)	IHC	Mouse
Janardhan KS, Jensen H, Clayton NP, Herbert RA Immunohistochemistry in Investigative and Toxicologic Pathology Toxicol Pathol. 7/1/2018 [PMID: 29966501] Details: Citation used the HRP format of this antibody.
H Lu, S Chelvanamb, C Poirier, J Saliba, KL March, M Clauss, NV Bogatcheva EMAPII Monoclonal Antibody Ameliorates Influenza A Virus-Induced Lung Injury Mol. Ther., 2018-06-15;0(0):. 2018-06-15 [PMID: 29910176] (Western Blot, Mouse)	Western Blot	Mouse
C Draijer, P Robbe, CE Boorsma, MN Hylkema, BN Melgert Dual role of YM1+ M2 macrophages in allergic lung inflammation Sci Rep, 2018-03-23;8(1):5105. 2018-03-23 [PMID: 29572536] (IHC-P, Mouse)	IHC-P	Mouse
J Dong, Q Ma Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis Nanotoxicology, 2018-01-16;0(0):1-16. 2018-01-16 [PMID: 29338488] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury Proc. Natl. Acad. Sci. U.S.A., 2016-11-28;113(50):E8151-E8158. 2016-11-28 [PMID: 27911817] (Western Blot, Mouse)	Western Blot	Mouse
Laurie P Shornick Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20 Adv Wound Care (New Rochelle), 2016-11-01;5(11):486-494. 2016-11-01 [PMID: 27867753] (IHC, Mouse)	IHC	Mouse
Severe Reduction in Number and Function of Peripheral T Cells Does Not Afford Protection toward Emphysema and Bronchial Remodeling Induced in Mice by Cigarette Smoke Am J Pathol, 2016-05-06;0(0):. 2016-05-06 [PMID: 27157991] (IHC-P, Mouse)	IHC-P	Mouse
Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema. PLoS ONE, 2015-03-19;10(3):e0119797. 2015-03-19 [PMID: 25790295] (IHC, Mouse)	IHC	Mouse
Guo JY, Karsli-Uzunbas G, Mathew R et al. Autophagy suppresses progression of K-ras-induced lung tumors to oncocytomas and maintains lipid homeostasis. Genes Dev 7/1/2013 [PMID: 23824538]
Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 2012-07-10;7(0):87-104. 2012-07-10 [PMID: 22837640] (Western Blot, Mouse)	Western Blot	Mouse
Muller U, Piehler D, Stenzel W, Kohler G, Frey O, Held J, Grahnert A, Richter T, Eschke M, Kamradt T, Brombacher F, Alber G Lack of IL-4 receptor expression on T helper cells reduces T helper 2 cell polyfunctionality and confers resistance in allergic bronchopulmonary mycosis. Mucosal Immunol, 2012-02-15;5(3):299-310. 2012-02-15 [PMID: 22333910] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Josefowicz SZ, Niec RE, Kim HY Extrathymically generated regulatory T cells control mucosal TH2 inflammation. Nature, 2012-02-08;482(7385):395-9. 2012-02-08 [PMID: 22318520] (IHC, Mouse)	IHC	Mouse
Asif M Qureshi, Adele Hannigan, Donald Campbell, Colin Nixon, Joanna B Wilson Chitinase-Like Proteins Are Autoantigens in a Model of Inflammation-Promoted Incipient Neoplasia Genes & Cancer 2011-01-01 [PMID: 21779482]
Reece JJ, Siracusa MC, Southard TL, Brayton CF, Urban JF, Scott AL Hookworm-induced persistent changes to the immunological environment of the lung. Infect. Immun., 2008-05-27;76(8):3511-24. 2008-05-27 [PMID: 18505812] (IHC-P, Mouse)	IHC-P	Mouse
Show All 25 Publications. Collapse Publications.

	Branislav Krljanac 07/16/2017
Application:	IHC-Fr
Species:	Mouse

Gene Symbol	CHI3L2
Uniprot	O35744()