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WRN Antibody

Images

 
Western Blot: WRN Antibody [NBP2-20914] - Sample (30 ug of whole cell lysate) A: A431 B: Molt-4 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: WRN Antibody [NBP2-20914] - Analysis of paraformaldehyde-fixed HeLa, antibody at 1:100.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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WRN Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human WRN. The exact sequence is proprietary.
Localization
Nucleus; nucleolus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WRN
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Western Blot 1:500 - 1:3000
Theoretical MW
162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-20914 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS 40% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for WRN Antibody

  • DKFZp686C2056
  • DNA helicase, RecQ-like type 3
  • EC 3.1
  • EC 3.1.-.-
  • EC 3.6.1
  • EC 3.6.4.12
  • Exonuclease WRN
  • RecQ protein-like 2
  • RecQ3
  • RECQ3RECQL3
  • RECQL2
  • RECQL2Werner syndrome ATP-dependent helicase
  • Werner syndrome
  • Werner syndrome, RecQ helicase-like
  • WRN

Background

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for WRN Antibody (NBP2-20914) (0)

There are no publications for WRN Antibody (NBP2-20914).
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Review for WRN Antibody (NBP2-20914) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-20914:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot WRN NBP2-20914
Enlarge
5
reviewed by:
Verified Customer
WB Human 07/25/2019
View

Summary

ApplicationWestern Blot
Sample TestedCancer cell lysates
SpeciesHuman
Lot39645

Comments

Comments40ug protein
1/1000 dilution Ab
incubation 4°C ON

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WRN Antibody (NBP2-20914) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional WRN Products

Array NBP2-20914

Research Areas for WRN Antibody (NBP2-20914)

Find related products by research area.

Blogs on WRN.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
07/25/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol WRN
Uniprot