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Wnt-5a Antibody (CL13683)

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Staining of human salivary gland shows moderate cytoplasmic positivity in glandular cells.
Staining of human salivary gland shows moderate cytoplasmic positivity in glandular cells.
Staining of human cerebellum shows no positivity in Purkinje cells as expected.
Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clone
CL13683
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Wnt-5a Antibody (CL13683) Summary

Immunogen
This antibody was designed to target the recombinant protein Wnt-5a, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
WNT5A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Wnt-5a Antibody (CL13683)

  • hWNT5A
  • protein Wnt-5a
  • wingless-type MMTV integration site family, member 5A
  • WNT-5A protein
  • Wnt5a
  • Wnt-5a

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Wnt-5a Antibody
(CL13683)
NBP3-24567
Wnt-5a Antibody (CL13683)
Species: Hu, Mu, Rt
Applications: IHC

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Secondary Antibodies

 

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Blogs on Wnt-5a.

Check out the latest blog posts on Wnt-5a.
The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.
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Bioinformatics

Gene Symbol WNT5A