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Wnt-11 Antibody [Unconjugated]

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Wnt-11 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Mouse Wnt-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2647) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Wnt-11 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Mouse Wnt-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2647) at 10 µg/mL for 3 hours at room temperature. ...read more
Hypoxia induces expression of WNT11 through VHL.(A,B) Higher basal levels of WNT11 protein in Vhl-deleted cells (lenti-Cre infected Vhlf/f). EMSCs isolated from Vhlf/f mouse were infected with lentivirus carrying either ...read more
WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO ...read more
WNT11 regulates MMPs activities.(A–C) (Top panels): Serum-free medium was conditioned for 24 hrs by the indicated cells, concentrated 20-fold and assayed by gelatin zymography. Gelatinolytic activity is indicated by ...read more
Induced WNT11 expression with tumor hypoxia and WNT11 regulates tumor growth.Antiangiogenic therapy induces WNT11 expression in the orthotopic malignant glioma model. Athymic mice implanted with U87 delta EGFR cells ...read more
HIF-1 alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia.(A,C–E) EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2647-SP
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Wnt-11 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Wnt‑11 peptide
Leu39-Ala79 and Lys225-Arg297
Accession # Q059Y4
Specificity
Detects mouse Wnt-11 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WNT11
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2647 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-11 Antibody [Unconjugated]

  • HWNT11
  • MGC141946
  • MGC141948
  • protein Wnt-11
  • wingless-type MMTV integration site family, member 11
  • Wnt11
  • Wnt-11

Background

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cell signaling molecules that play important roles in vertebrate development. Mouse Wnt-11 shares 97% amino acid sequence identity with its human and rat homolog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Wnt-11 Antibody
[Unconjugated]
AF2647
Wnt-11 Antibody [Unconjugated]
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Publications for Wnt-11 Antibody (AF2647)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Product General Protocols

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Bioinformatics

Gene Symbol WNT11
Uniprot