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WIF-1 Antibody (133015) [Unconjugated]

 (3 Publications)
 (1 Review)
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WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
133015
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB134-500
Carrier Free
MAB134-SP
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WIF-1 Antibody (133015) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human WIF-1
Gly29-Trp379
Accession # AAD25402
Specificity
Detects human WIF-1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse WIF‑1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
WIF1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB134 in the following applications:
Publications
Read Publications using
MAB134 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WIF-1 Antibody (133015) [Unconjugated]

  • WIF1
  • WIF-1
  • WNT inhibitory factor 1

Background

WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WIF-1 Antibody
(133015) [Unconjugate
MAB134
WIF-1 Antibody (133015) [Unconjugated]
Species: Hu
Applications: WB, IHC

Publications for WIF-1 Antibody (MAB134)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-Fr, IHC-P.


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IHC-Fr
(1)
IHC-P
(2)
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Human
(3)
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Review for WIF-1 Antibody (MAB134) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB134:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot WIF-1 MAB134
Enlarge 		4
reviewed by:
Verified Customer
 WB Human 02/19/2019
View

Summary

ApplicationWestern Blot
Sample TestedHCT116 cell line lysate
SpeciesHuman
LotEZP0416081

Comments

CommentsHuman HCT116 cells transfected with WIF1 expression vector for 24 hours.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for WIF-1 Antibody (MAB134) (0)

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Secondary Antibodies

 

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Calculators

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Recent Reviews

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Western Blot WIF-1 MAB134
Enlarge
 Verified Customer
02/19/2019
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol WIF1
Uniprot