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WDR77 Antibody

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Western Blot: WDR77 Antibody [NB100-77305] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-WDR77/MEP50 antibody sed for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WDR77/MEP50 was also immunoprecipitated by rabbit anti-WDR77/MEP50 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).
Western Blot: WDR77 Antibody [NB100-77305] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-WDR77/MEP50 antibody ...read more
Immunohistochemistry: WDR77 Antibody [NB100-77305] - Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti- WDR77/MEP50 used at a dilution of 1:1,000 (0.2ug/ml). Detection: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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WDR77 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 292 and 342 of human WD repeat domain 77 (methylosome protein 50) using the numbering given in entry NP_077007.1 (GeneID 79084).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WDR77
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5ug/mg
  • Western Blot 1:100-1:2000
Application Notes
Suitable for Immunofluorescence, Immunohistochemistry-Paraffin, Western blotting and Immunoprecipitation.
Control
WDR77 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WDR77 Antibody

  • Androgen receptor cofactor p44
  • MEP-50
  • MEP50Nbla10071
  • methylosome protein 50
  • MGC2722
  • p44
  • p44/Mep50
  • RP11-552M11.3
  • WD repeat domain 77
  • WD repeat-containing protein 77

Background

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Control Lysate(s)

WDR77 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WDR77
Entrez
Uniprot