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VPS54 Antibody

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Immunocytochemistry/Immunofluorescence: VPS54 Antibody [NBP2-57640] - Staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
Immunocytochemistry/ Immunofluorescence: VPS54 Antibody [NBP2-57640] - Staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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VPS54 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: EKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVN
Predicted Species
Mouse (97%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS54
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
VPS54 Recombinant Protein Antigen (NBP2-57640PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for VPS54 Antibody

  • HCC8SLP-8p
  • Hepatocellular carcinoma protein 8
  • hVps54L
  • Tumor antigen HOM-HCC-8
  • Tumor antigen SLP-8p
  • vacuolar protein sorting 54 (yeast)
  • vacuolar protein sorting 54 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 54
  • VPS54L

Background

VPS54 encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional VPS54 Products

Array NBP2-57640

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Bioinformatics

Gene Symbol VPS54