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VPS37A Antibody

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Western Blot: VPS37A Antibody [NBP1-83528] - Analysis in rat cell line NBT-II and mouse cell line NIH-3T3.
Immunocytochemistry/ Immunofluorescence: VPS37A Antibody [NBP1-83528] - Staining of human cell line U-251 MG shows localization to cytosol, centrosome and vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VPS37A Antibody [NBP1-83528] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Western Blot: VPS37A Antibody [NBP1-83528] - Analysis in human testis tissue.
Immunohistochemistry-Paraffin: VPS37A Antibody [NBP1-83528] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: VPS37A Antibody [NBP1-83528] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: VPS37A Antibody [NBP1-83528] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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VPS37A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DVPDAFPELSELSVSQLTDMNEQEEVLLEQFLTLPQLKQIITDKDDLVKSIEELARKNLLLEPSLEAKRQTVLDKYELLTQMKSTF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS37A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
VPS37A Protein (NBP1-83528PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VPS37A Antibody

  • ESCRT-I complex subunit VPS37A
  • FLJ32642
  • HCRP1FLJ42616
  • hepatocellular carcinoma related protein 1
  • Hepatocellular carcinoma-related protein 1
  • hVps37A
  • polyglutamine binding protein 2
  • PQBP2
  • vacuolar protein sorting 37 homolog A (S. cerevisiae)
  • vacuolar protein sorting 37A (yeast)
  • vacuolar protein sorting 37A
  • vacuolar protein sorting-associated protein 37A

Background

Component of the escrt-i complex, a regulator of vesicular trafficking process. required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. may be involved in cell growth and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VPS37A Antibody (NBP1-83528) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS37A