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FAM125A Antibody

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Western Blot: FAM125A Antibody [NBP1-91882] - Analysis in human cell line BEWO.
Immunocytochemistry/ Immunofluorescence: FAM125A Antibody [NBP1-91882] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
Immunohistochemistry-Paraffin: FAM125A Antibody [NBP1-91882] - Staining of human Small intestine shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: FAM125A Antibody [NBP1-91882] - Staining of human Heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: FAM125A Antibody [NBP1-91882] - Staining of human Skin shows strong membranous and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: FAM125A Antibody [NBP1-91882] - Staining of human Urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FAM125A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LGATDTAVFDVRLSGKTKTVPGYLRIGDMGGFAIWCKKAKAPRPVPKPRGLSRDMQGLSLDAASQPSKGGLLERTASRLGSRASTL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MVB12A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FAM125A Protein (NBP1-91882PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FAM125A Antibody

  • CFBP
  • CIN85/CD2AP family binding protein
  • CIN85/CD2AP family-binding protein
  • ESCRT-I complex subunit MVB12A
  • family with sequence similarity 125, member A
  • FLJ32495
  • multivesicular body subunit 12A
  • Protein FAM125A

Background

FAM125A is a component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting ofendocytic ubiquitinated cargos into multivesicular bodies. May be involved in the ligand-mediated internalization anddown-regulation of EGF receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MVB12A