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Vinculin Antibody (728513) [Unconjugated]

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Vinculin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB68961) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB68961) at 15 µg/mL overnight at 4 °C. Before incubation with the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC, ICC/IF
Clone
728513
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Vinculin Antibody (728513) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Vinculin
Lys1020-Gln1134
Accession # P18206
Specificity
Detects human Vinculin in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
VCL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publication using
MAB68961 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vinculin Antibody (728513) [Unconjugated]

  • CMD1W
  • CMH15
  • metavinculin
  • MVCL
  • VCL
  • Vinculin

Background

VCL (vinculin) is a 117 kDa actin binding cytoskeletal phosphoprotein found in adherens-type cell-cell and cell-matrix junctions. The 1066 amino acid (aa) human vinculin contains an N-terminal globular head (aa 2-835) with a talin interaction domain (aa 168-208) and three tandem repeats (aa 259-589), a pro-rich linker region (aa 826-878), and a C-terminal tail that facilitates phospholipid membrane insertion (aa 1003-1134). An 1134 aa form termed metavinculin contains 68 aa inserted after aa 915 and mainly occurs in cardiac, skeletal and smooth muscle. Human, mouse and rat vinculin share 100% aa sequence identity within the region used as an immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Vinculin Antibody (MAB68961)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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Reviews for Vinculin Antibody (MAB68961) (0)

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FAQs for Vinculin Antibody (MAB68961). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.
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Bioinformatics

Gene Symbol VCL
Uniprot