Home » VIAAT/SLC32A1/VGAT » VGAT Antibodies » VIAAT/SLC32A1/VGAT Antibody (731307) [Unconjugated]
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VIAAT/SLC32A1/VGAT Antibody (731307) [Unconjugated]

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VIAAT/SLC32A1 was detected in immersion fixed 7-day differentiated rat cortical stem cells using Mouse Anti-Human VIAAT/SLC32A1 Monoclonal Antibody (Catalog # MAB6847) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
VIAAT/SLC32A1 was detected in immersion fixed 7-day differentiated rat cortical stem cells using Mouse Anti-Human VIAAT/SLC32A1 Monoclonal Antibody (Catalog # MAB6847) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
731307
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6847-SP
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VIAAT/SLC32A1/VGAT Antibody (731307) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VIAAT/SLC32A1
Ala2-Phe133
Accession # Q9H598
Specificity
Detects human VIAAT/SLC32A1 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC32A1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using MAB6847.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VIAAT/SLC32A1/VGAT Antibody (731307) [Unconjugated]

  • bA122O1.1
  • hVIAAT
  • SLC32A1
  • solute carrier family 32 (GABA vesicular transporter), member 1
  • Solute carrier family 32 member 1
  • Vesicular GABA transporter
  • vesicular inhibitory amino acid transporter
  • VGAT
  • VGATGABA and glycine transporter
  • VIAAT

Background

VIAAT (Vesicular inhibitory amino acid transporter; also VGAT and SLC32A1) is a 56-58 kDa member of the amino acid/polyamine transporter 2 family of proteins. It is expressed in inhibitory neurons throughout the CNS, in both resting and activated lymphocytes, and in pancreatic alpha -cells (in rodent). VIAAT is believed to transport both GABA and glycine across synaptic vesicle membranes in an electrical and pH gradient-dependent manner. Once transported, these neurotransmitters are released at the presynaptic membrane, acting as inhibitory factors in the mature nervous system, and excitatory factors in the immature nervous system. Human VIAAT is a 525 amino acid (aa) 10-transmembrane nonglycosylated protein. The N-terminus (aa 1-133) and C-terminus (aa 511-525) are cytoplasmic. There is a 52 kDa short form that is not well characterized. Over aa 2-133, human VIAAT shares 95% aa identity with mouse VIAAT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VIAAT/SLC32A1/VGAT Antibody (MAB6847)(1)


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Bioinformatics

Gene Symbol SLC32A1
Uniprot