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Human VEGF 165 ELISA Kit (Colorimetric)

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ELISA: Human VEGF 165 ELISA Kit (Colorimetric) [NBP2-79681] - Samples were spiked with high concentrations of Human VEGF 165 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Human VEGF 165 ELISA Kit (Colorimetric) [NBP2-79681] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, and other biological fluids.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

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Human VEGF 165 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human VEGF165 in samples. No Significant cross-reactivity or interference between Human VEGF165 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 4.79
Intra-Assay
%CV < 4.65
Spike Recovery
83 - 107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
VEGFA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human VEGF 165 ELISA Kit (Colorimetric)

  • biosimilar antibody
  • biosimilars
  • MVCD1
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • VEGF-A
  • VEGFMGC70609
  • VPFvascular endothelial growth factor

Background

VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol VEGFA