![Western Blot: VDAC1 Antibody (6B1K1) [NBP3-15871] - Analysis of extracts of various cell lines, using VDAC1 antibody (NBP3-15871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/VDAC1-Antibody-6B1K1-Western-Blot-NBP3-15871-img0003.jpg "Western Blot: VDAC1 Antibody (6B1K1) [NBP3-15871] - Analysis of extracts of various cell lines, using VDAC1 antibody (NBP3-15871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 6B1K1 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VDAC1 (P21796). MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTETTKVTGSLETKYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLTFD |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | VDAC1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.05% BSA, 50% glycerol, pH7.3 |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for VDAC1 Antibody (NBP3-15871)Find related products by research area.
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| Gene Symbol | VDAC1 |
![Immunocytochemistry/Immunofluorescence: VDAC1 Antibody (6B1K1) [NBP3-15871] - Immunofluorescence analysis of L929 cells using VDAC1 antibody (NBP3-15871). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VDAC1-Antibody-6B1K1-Immunocytochemistry-Immunofluorescence-NBP3-15871-img0002.jpg "Immunocytochemistry/Immunofluorescence: VDAC1 Antibody (6B1K1) [NBP3-15871] - Immunofluorescence analysis of L929 cells using VDAC1 antibody (NBP3-15871). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] - Human colon carcinoma tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-immunohistochemistry-paraffin-492024102517..jpg "Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] - Human colon carcinoma tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -Human liver tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-immunohistochemistry-paraffin-492024102614..jpg "Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -Human liver tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -Human thyroid cancer tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-immunohistochemistry-paraffin-492024102714..jpg "Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -Human thyroid cancer tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -Mouse kidney tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15871_rabbit-vdac1-mab-6b1k1-immunohistochemistry-paraffin-492024102816..jpg "Immunohistochemistry-Paraffin: VDAC1 Antibody (6B1K1) [NBP3-15871] -Mouse kidney tissue using VDAC1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")