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VASA Antibody (246904) [Unconjugated]

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Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VASA Monoclonal Antibody (Catalog # MAB2030) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VASA Monoclonal Antibody (Catalog # MAB2030) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
246904
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2030-SP
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VASA Antibody (246904) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0
Specificity
Detects human VASA in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DDX4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using MAB2030.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VASA Antibody (246904) [Unconjugated]

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

VASA, also known as DDX4, is a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is a highly specific marker of germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VASA Antibody (MAB2030)(1)


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Bioinformatics

Gene Symbol DDX4
Uniprot