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V-type proton ATPase subunit F Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

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V-type proton ATPase subunit F Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Produced in rabbits immunized with purified, recombinant Human V-type proton ATPase subunit F (Accession#: NP_004222.2; Met1-Arg119)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1F
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen and protein A Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for V-type proton ATPase subunit F Antibody [CoraFluor™ 1]

  • ATP6S14adenosinetriphosphatase 14k chain
  • ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
  • H(+)-transporting two-sector ATPase, 14kD subunit
  • MGC117321
  • MGC126037
  • MGC126038
  • vacuolar ATP synthase subunit F
  • vacuolar proton pump F subunit
  • Vacuolar proton pump subunit F
  • VATFATPase, vacuolar, 14 kD
  • V-ATPase 14 kDa subunit
  • V-ATPase F subunit
  • V-ATPase subunit F
  • Vma7
  • V-type proton ATPase subunit F

Background

V-type proton ATPase subunit F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATP6V1F