Novus Biologicals products are now on bio-techne.com

V-type proton ATPase subunit F Antibody (OTI1B8) [Janelia Fluor® 525]

Images

 
There are currently no images for V-type proton ATPase subunit F Antibody (NBP2-74863JF525).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

V-type proton ATPase subunit F Antibody (OTI1B8) [Janelia Fluor® 525] Summary

Immunogen
Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATP6V1F
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for V-type proton ATPase subunit F Antibody (OTI1B8) [Janelia Fluor® 525]

  • ATP6S14adenosinetriphosphatase 14k chain
  • ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
  • H(+)-transporting two-sector ATPase, 14kD subunit
  • MGC117321
  • MGC126037
  • MGC126038
  • vacuolar ATP synthase subunit F
  • vacuolar proton pump F subunit
  • Vacuolar proton pump subunit F
  • VATFATPase, vacuolar, 14 kD
  • V-ATPase 14 kDa subunit
  • V-ATPase F subunit
  • V-ATPase subunit F
  • Vma7
  • V-type proton ATPase subunit F

Background

V-type proton ATPase subunit F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-01991
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-30993
Species: Ha, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-31944
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-55232
Species: Hu
Applications: ICC/IF, WB
NB300-270
Species: Ch, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, In vitro, Simple Western, WB
NBP1-32980
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC-WhMt, IHC, IHC-P, WB
NBP1-88894
Species: Hu
Applications: IHC, IHC-P
H00009114-M01
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP1-59654
Species: Hu
Applications: WB
NBP2-83943
Species: Rt
Applications: WB
H00000534-M02
Species: Hu, Mu, Rt
Applications: ELISA, WB
NBP2-15520
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-19506
Species: Hu, Rt
Applications: IHC, IHC-P, WB
H00028299-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-84475
Species: Hu, Mu
Applications: IHC, IHC-P, WB
H00000537-M01
Species: Hu, Rt
Applications: ELISA, S-ELISA, WB
NBP1-59922
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-74863JF525
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

Publications for V-type proton ATPase subunit F Antibody (NBP2-74863JF525) (0)

There are no publications for V-type proton ATPase subunit F Antibody (NBP2-74863JF525).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for V-type proton ATPase subunit F Antibody (NBP2-74863JF525) (0)

There are no reviews for V-type proton ATPase subunit F Antibody (NBP2-74863JF525). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for V-type proton ATPase subunit F Antibody (NBP2-74863JF525) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional V-type proton ATPase subunit F Products

Blogs on V-type proton ATPase subunit F

There are no specific blogs for V-type proton ATPase subunit F, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our V-type proton ATPase subunit F Antibody (OTI1B8) [Janelia Fluor® 525] and receive a gift card or discount.

Bioinformatics

Gene Symbol ATP6V1F