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UTP18 Antibody

Images

 
Western Blot: UTP18 Antibody [NBP2-13511] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: UTP18 Antibody [NBP2-13511] - Staining of human cell line A-431 shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: UTP18 Antibody [NBP2-13511] - Staining of human cerebral cortex shows strong nucleolar positivity in neurons.
Immunocytochemistry/ Immunofluorescence: UTP18 Antibody [NBP2-13511] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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UTP18 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: HEDSGDSEVENEAKGNFPPQKKPVWVDEEDEDEEMVDMMNNRFRKDMMKNASESKLSKDNLKKRLKEEFQHAMGGVPAWAETTKRKT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UTP18
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Immunoprecipitation
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
UTP18 Protein (NBP2-13511PEP)
Publications
Read Publication using
NBP2-13511 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%). Human reactivity reported in scientific literature (PMID: 25435373).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UTP18 Antibody

  • CGI-48
  • U3 small nucleolar RNA-associated protein 18 homolog
  • UTP18, small subunit (SSU) processome component, homolog (yeast)
  • WD repeat domain 50
  • WD repeat-containing protein 50
  • WDR50

Background

UTP18/WDR50 is a component of the small-subunit (SSU) processome, a large ribonucleoprotein complex that participates in ribosomal RNA processing and ribosome assembly. UTP18/WDR50 is a member of the WD repeat family. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UTP18 Antibody (NBP2-13511)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IP, WB.


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ICC/IF
(1)
IF/IHC
(1)
IP
(1)
WB
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Human
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UTP18 Antibody (NBP2-13511) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-13511

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Bioinformatics

Gene Symbol UTP18