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EMG1 Antibody (OTI1B8) - Azide and BSA Free

Images

 
Western Blot: EMG1 Antibody (OTI1B8) - Azide and BSA Free [NBP2-71601] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EMG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: EMG1 Antibody (OTI1B8) - Azide and BSA Free [NBP2-71601] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1.
Flow Cytometry: EMG1 Antibody (OTI1B8) - Azide and BSA Free [NBP2-71601] - Analysis of Jurkat cells, using anti-EMG1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: EMG1 Antibody (OTI1B8) - Azide and BSA Free [NBP2-71601] - Analysis of extracts (35ug) from 9 different cell lines by using anti-EMG1 monoclonal antibody.
Flow Cytometry: EMG1 Antibody (OTI1B8) - Azide and BSA Free [NBP2-71601] - Analysis of Hela cells, using anti-EMG1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
OTI1B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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EMG1 Antibody (OTI1B8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human EMG1(NP_006322) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EMG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-2000
Theoretical MW
26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for EMG1 Antibody (OTI1B8) - Azide and BSA Free

  • 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1
  • BWCNS
  • C2F18S rRNA Psi1248 methyltransferase
  • EMG1 nucleolar protein homolog (S. cerevisiae)
  • essential for mitotic growth 1
  • FLJ60792
  • Grcc2f
  • NEP1EC 2.1.1.-
  • Nucleolar protein EMG1 homolog
  • probable ribosome biogenesis protein NEP1
  • Protein C2f
  • Ribosome biogenesis protein NEP1

Background

EMG1 plays a role in the processing of the pre-18S rRNA and small ribosomal subunit assembly (Liu and Thiele, 2001 [PubMed 11694595]) and may play a role in methylation during ribosome biogenesis (Eschrich et al., 2002 [PubMed 11935223]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EMG1 Antibody (NBP2-71601) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EMG1