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USP32 Antibody

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Western Blot: USP32 Antibody [NBP2-13509] - Analysis in human cell line MCF-7.
Western Blot: USP32 Antibody [NBP2-13509] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: USP32 Antibody [NBP2-13509] - Staining of human cell line MCF7 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: USP32 Antibody [NBP2-13509] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: USP32 Antibody [NBP2-13509] - Analysis in human cell line MCF-7 and human cell line HEK 293.
Immunohistochemistry-Paraffin: USP32 Antibody [NBP2-13509] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: USP32 Antibody [NBP2-13509] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: USP32 Antibody [NBP2-13509] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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USP32 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: VVIEPSSVLNGGKYSFGTAAHPMEQVEDRIGSSLSYVNTTEEKFSDNISTASEASETAGSGFLYSATPGADVCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPE
Predicted Species
Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP32
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
USP32 Protein (NBP2-13509PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for USP32 Antibody

  • Deubiquitinating enzyme 32
  • EC 3.1.2.15
  • EC 3.4.19.12
  • NY-REN-60
  • Renal carcinoma antigen NY-REN-60
  • ubiquitin carboxyl-terminal hydrolase 32
  • ubiquitin specific peptidase 32
  • ubiquitin specific protease 32
  • ubiquitin thioesterase 32
  • Ubiquitin thiolesterase 32
  • USP10Ubiquitin-specific-processing protease 32
  • USP32

Background

Ubiquitin-specific peptidase 32 (USP32) is a thioesterase enzyme that catalyzes the hydrolysis of ubiquitin from ubiquitin conjugates. USP32 has also been identified as an antigen recognized by autologous antibodies in patients with renal-cell carcinoma and as part of a chimeric gene, the Tre2 (USP6) oncogene, which resulted from a fusion between USP32 and TBC1D3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol USP32