![Western Blot: USP10 Antibody (OTI2E1) [NBP2-01452] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and USP10-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-USP10 monoclonal antibody NBP2-01452, (1:500). Then the blotted membrane was stripped and reprobed with an anti-beta-actin antibody as a loading control.](http://images.novusbio.com/fullsize/USP10-Antibody-OTI2E1-Western-Blot-NBP2-01452-img0013.jpg "Western Blot: USP10 Antibody (OTI2E1) [NBP2-01452] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and USP10-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-USP10 monoclonal antibody NBP2-01452, (1:500). Then the blotted membrane was stripped and reprobed with an anti-beta-actin antibody as a loading control.")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, KO |
| Clone | OTI2E1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human USP10(NP_005144) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | USP10 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 87 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-01452 | Applications | Species |
|---|---|---|
| Xia X, Liu X, Chai R et al. USP10 exacerbates neointima formation by stabilizing Skp2 protein in vascular smooth muscle cells Journal of Biological Chemistry 2021-09-01 [PMID: 34599966] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for USP10 Antibody (NBP2-01452)Find related products by research area.
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Beclin 1: Regulator of Autophagy and Apoptosis Beclin 1 is the mammalian orthologue of the yeast Apg6/Vps30 gene. Beclin 1 can complement the defect in autophagy present in apg6 yeast strains and stimulate autophagy when overexpressed in mammalian cells (1) and can bind to Bcl2, an important regul... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: USP10 Antibody (2E1) [NBP2-01452] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Immunocytochemistry-Immunofluorescence-NBP2-01452-img0010.jpg "Immunocytochemistry/Immunofluorescence: USP10 Antibody (2E1) [NBP2-01452] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10.")
![Immunohistochemistry-Paraffin: USP10 Antibody (OTI2E1) [NBP2-01452] - Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-01452)](http://images.novusbio.com/fullsize/USP10-Antibody-OTI2E1-Immunohistochemistry-Paraffin-NBP2-01452-img0012.jpg "Immunohistochemistry-Paraffin: USP10 Antibody (OTI2E1) [NBP2-01452] - Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-01452)")
![Flow Cytometry: USP10 Antibody (2E1) [NBP2-01452] - Analysis of Hela cells, using anti-USP10 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Flow-Cytometry-NBP2-01452-img0001.jpg "Flow Cytometry: USP10 Antibody (2E1) [NBP2-01452] - Analysis of Hela cells, using anti-USP10 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: USP10 Antibody (2E1) [NBP2-01452] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP10.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Western-Blot-NBP2-01452-img0002.jpg "Western Blot: USP10 Antibody (2E1) [NBP2-01452] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP10.")
![Western Blot: USP10 Antibody (2E1) [NBP2-01452] Analysis of extracts (35ug) from 9 different cell lines by using anti-USP10 monoclonal antibody.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Western-Blot-NBP2-01452-img0011.jpg "Western Blot: USP10 Antibody (2E1) [NBP2-01452] Analysis of extracts (35ug) from 9 different cell lines by using anti-USP10 monoclonal antibody.")
![Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Immunohistochemistry-Paraffin-NBP2-01452-img0003.jpg "Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP10 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Immunohistochemistry-Paraffin-NBP2-01452-img0004.jpg "Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human breast tissue using anti-USP10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Immunohistochemistry-Paraffin-NBP2-01452-img0005.jpg "Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human breast tissue using anti-USP10 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human endometrium tissue using anti-USP10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Immunohistochemistry-Paraffin-NBP2-01452-img0006.jpg "Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human endometrium tissue using anti-USP10 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human lymphoma tissue using anti-USP10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Immunohistochemistry-Paraffin-NBP2-01452-img0007.jpg "Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human lymphoma tissue using anti-USP10 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human pancreas tissue using anti-USP10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Immunohistochemistry-Paraffin-NBP2-01452-img0008.jpg "Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human pancreas tissue using anti-USP10 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human prostate tissue using anti-USP10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP10-Antibody-2E1-Immunohistochemistry-Paraffin-NBP2-01452-img0009.jpg "Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human prostate tissue using anti-USP10 mouse monoclonal antibody.")
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg)
![Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg)
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")