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CBL Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: CBL Antibody [NBP1-86588] - Analysis in human testis and skeletal muscle tissues. Corresponding CBL RNA-seq data are presented for the same tissues.
Western Blot: CBL Antibody [NBP1-86588] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: CBL Antibody [NBP1-86588] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: CBL Antibody [NBP1-86588] - Staining of human tonsil shows strong membranous positivity in germinal center cells.
Western Blot: CBL Antibody [NBP1-86588] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-103
Immunohistochemistry-Paraffin: CBL Antibody [NBP1-86588] - Staining of human colon shows strong membranous positivity in lymphoid cells.
Immunohistochemistry-Paraffin: CBL Antibody [NBP1-86588] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: CBL Antibody [NBP1-86588] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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CBL Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CBL Protein (NBP1-86588PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CBL Antibody

  • Cas-Br-M (murine) ecotropic retroviral transforming sequence
  • Casitas B-lineage lymphoma proto-oncogene
  • CBL
  • CBL2
  • CBL2E3 ubiquitin-protein ligase CBL
  • EC 6.3.2
  • EC 6.3.2.-
  • oncogene CBL2
  • Proto-oncogene c-Cbl
  • RING finger protein 55
  • RNF55
  • RNF55C-CBL
  • Signal transduction protein CBL

Background

c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CBL Antibody (NBP1-86588) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CBL Products

Array NBP1-86588

Research Areas for CBL Antibody (NBP1-86588)

Find related products by research area.

Blogs on CBL.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Bioinformatics

Gene Symbol CBL