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UBA52 Antibody (OTI4F2) - Azide and BSA Free

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Western Blot: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBA52.
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: UBA52 Antibody (OTI4F2) - Azide and BSA Free [NBP2-74730] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clone
OTI4F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

UBA52 Antibody (OTI4F2) - Azide and BSA Free Summary

Immunogen
Synthetic peptide corresponding to residues near C-terminus of human UBA52 (NP_003324).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
UBA52
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for UBA52 Antibody (OTI4F2) - Azide and BSA Free

  • CEP52ubiquitin carboxyl extension protein 52
  • HUBCEP52
  • MGC126879
  • MGC126881
  • MGC57125
  • RPL40
  • UBCEP2
  • ubiquitin A-52 residue ribosomal protein fusion product 160S ribosomal protein L40
  • ubiquitin-52 amino acid fusion protein
  • ubiquitin-60S ribosomal protein L40
  • ubiquitin-CEP52

Background

UBA52 - ubiquitin A-52 residue ribosomal protein fusion product 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBA52