![Immunohistochemistry-Paraffin: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - RPL32 antibody [HL2329] detects RPL32 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. RPL32 stained by RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25485_rabbit-rpl32-mab-hl2329-azide-and-bsa-free-2120249393568.jpg "Immunohistochemistry-Paraffin: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - RPL32 antibody [HL2329] detects RPL32 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. RPL32 stained by RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | Hu, Mu, Rt, Ca, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL2329 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Full length human RPL32 recombinant protein. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RPL32 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 16 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RPL32 |
![Western Blot: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25485_rabbit-rpl32-mab-hl2329-azide-and-bsa-free-2120249362798.jpg "Western Blot: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - RPL32 antibody [HL2329] detects RPL32 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPL32 stained by RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25485_rabbit-rpl32-mab-hl2329-azide-and-bsa-free-2120249351342.jpg "Immunocytochemistry/Immunofluorescence: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - RPL32 antibody [HL2329] detects RPL32 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPL32 stained by RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .")
![Western Blot: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25485_rabbit-rpl32-mab-hl2329-azide-and-bsa-free-2120249403849.jpg "Western Blot: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25485_rabbit-rpl32-mab-hl2329-azide-and-bsa-free-212024940818.jpg "Western Blot: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - RPL32 antibody [HL2329] detects RPL32 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. RPL32 stained by RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25485_rabbit-rpl32-mab-hl2329-azide-and-bsa-free-2120249422626.jpg "Immunohistochemistry-Paraffin: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - RPL32 antibody [HL2329] detects RPL32 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. RPL32 stained by RPL32 antibody [HL2329] (NBP3-25485) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2329] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25485_rabbit-rpl32-mab-hl2329-azide-and-bsa-free-western-blot-16220249123..jpg "Western Blot: RPL32 Antibody (HL2329) - Azide and BSA Free [NBP3-25485] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody [HL2329] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")