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RPL8 Antibody

Images

 
Western Blot: RPL8 Antibody [NBP2-47297] - Analysis using Anti-RPL8 antibody NBP2-47297 (A) shows similar pattern to independent antibody NBP2-48827 (B).
Immunocytochemistry/ Immunofluorescence: RPL8 Antibody [NBP2-47297] - Staining of human cell line MCF7 shows localization to nucleoli, cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human cerebellum, fallopian tube, pancreas and skin using Anti-RPL8 antibody NBP2-47297 (A) shows similar protein distribution across tissues to ...read more
Independent Antibodies: Western Blot: RPL8 Antibody [NBP2-47297] - Analysis using Anti-RPL8 antibody NBP2-47297 (A) shows similar pattern to independent antibody NBP2-48827 (B).
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human lymph node.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human colon.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human liver.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human cerebral cortex.
Independent Antibodies: Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human cerebellum, fallopian tube, pancreas and skin using Anti-RPL8 antibody NBP2-47297 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: RPL8 Antibody [NBP2-47297] - Staining of human Fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

RPL8 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: VISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEK
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPL8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RPL8 Recombinant Protein Antigen (NBP2-47297PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPL8 Antibody

  • ribosomal protein L8,60S ribosomal protein L8

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPL8 Antibody (NBP2-47297) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on RPL8

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Bioinformatics

Gene Symbol RPL8