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RPL13 Antibody

Images

 
Western Blot: RPL13 Antibody [NBP1-57476] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: RPL13 Antibody [NBP1-57476] - Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasmic in alveolar mostly type I cells Primary Antibody Concentration: 1:100 Other ...read more
Western Blot: RPL13 Antibody [NBP1-57476] - Jurkat cell lysate, concentration 0.0625ug/ml.
Western Blot: RPL13 Antibody [NBP1-57476] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml RPL13 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.
Western Blot: RPL13 Antibody [NBP1-57476] - Hela, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that RPL13 is expressed in HeLa.
Western Blot: RPL13 Antibody [NBP1-57476] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.
Western Blot: RPL13 Antibody [NBP1-57476] - MCF7, Antibody Dilution: 1.0 ug/ml RPL13 is strongly supported by BioGPS gene expression data to be expressed in MCF7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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RPL13 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to RPL13 (ribosomal protein L13) The peptide sequence was selected from the C terminal of RPL13 (NP_000968). Peptide sequence KKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPL13
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1.0 ug/ml
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-57476.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for RPL13 Antibody

  • BBC1FLJ27454
  • Breast basic conserved protein 1
  • D16S444E
  • FLJ27453
  • MGC117342
  • MGC71373,60S ribosomal protein L13
  • OK/SW-cl.46
  • ribosomal protein L13

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL13 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Transcript variants derived from alternative splicing and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPL13 Antibody (NBP1-57476)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-57476 Applications Species
Bi,W. Genome Res. 12 (5), 713-728. 2002-01-01 [PMID: 11997338]

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RPL13
Entrez
Uniprot