![Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis using DDX39B mAb against human DDX39B (AA: 1-250) recombinant protein. (Expected MW is 54.2 kDa)](http://images.novusbio.com/fullsize/UAP56-Antibody-2F5G7-Western-Blot-NBP2-52455-img0006.jpg "Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis using DDX39B mAb against human DDX39B (AA: 1-250) recombinant protein. (Expected MW is 54.2 kDa)")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, CyTOF-ready |
| Clone | 2F5G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human UAP56 (AA: 1-250) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DDX39B |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DDX39B |
![Immunocytochemistry/Immunofluorescence: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of HeLa cells using DDX39B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/UAP56-Antibody-2F5G7-Immunofluorescence-NBP2-52455-img0001.jpg "Immunocytochemistry/Immunofluorescence: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of HeLa cells using DDX39B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
![Immunohistochemistry: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of paraffin-embedded HeLa tissues using DDX39B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/UAP56-Antibody-2F5G7-Immunohistochemistry-NBP2-52455-img0003.jpg "Immunohistochemistry: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of paraffin-embedded HeLa tissues using DDX39B mouse mAb with DAB staining.")
![Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Western blot analysis using DDX39B mAb against HEK293 (1) and DDX39B (AA: 1-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/UAP56-Antibody-2F5G7-Western-Blot-NBP2-52455-img0004.jpg "Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Western blot analysis using DDX39B mAb against HEK293 (1) and DDX39B (AA: 1-250)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis using DDX39B mouse mAb against HepG2 (1), HepG2 (2), K562 (3), Jurkat (4), NIH/3T3 (5), MCF-7 (6), Jurkat (7), and Hek293 (8) cell lysate.](http://images.novusbio.com/fullsize/UAP56-Antibody-2F5G7-Western-Blot-NBP2-52455-img0007.jpg "Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis using DDX39B mouse mAb against HepG2 (1), HepG2 (2), K562 (3), Jurkat (4), NIH/3T3 (5), MCF-7 (6), Jurkat (7), and Hek293 (8) cell lysate.")
![Immunofluorescence: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of HeLa cells using DDX39B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/UAP56-Antibody-2F5G7-Immunofluorescence-NBP2-52455-img0002.jpg "Immunofluorescence: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of HeLa cells using DDX39B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
![ELISA: UAP56 Antibody (2F5G7) [NBP2-52455] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/UAP56-Antibody-2F5G7-ELISA-NBP2-52455-img0008.jpg "ELISA: UAP56 Antibody (2F5G7) [NBP2-52455] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-PAGE Lymphotoxin-alpha/TNF-beta [Unconjugated]](https://images.novusbio.com/images2/Lymphotoxin-alpha_211-TBB_CF_695.jpg)
![Bioactivity Lymphotoxin-alpha/TNF-beta [Unconjugated]](https://images.novusbio.com/images2/Lymphotoxin-alpha_211-TBB_CF_697.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")