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U2AF35 Antibody

Images

 
Genetic Strategies: Western Blot: U2AF35 Antibody [NBP1-92553] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-92553] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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U2AF35 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MHLKPISRELRRELYGRRRKKHRSRS
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
U2AF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
U2AF35 Protein (NBP1-92553PEP)
Publications
Read Publication using NBP1-92553.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24579871)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for U2AF35 Antibody

  • FP793
  • human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712
  • RN
  • RNU2AF1,35-KD subunit
  • U2 auxiliary factor 35 kDa subunit
  • U2 small nuclear RNA auxiliary factor 1
  • U2 small nuclear RNA auxillary factor 1
  • U2 snRNP auxiliary factor small subunit

Background

U2AF35 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for U2AF35 Antibody (NBP1-92553)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for U2AF35 Antibody (NBP1-92553) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol U2AF1