U2AF35 Antibodies
We offer U2AF35 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each U2AF35 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our U2AF35 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the U2AF35 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our U2AF35 polyclonal antibodies and browse our U2AF35 monoclonal antibody catalog.
Alternate Names for U2AF35 Antibodies
anti-U2AF35 antibody, anti-U2AF1 antibody, anti-FP793 antibody, anti-human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712 antibody, anti-RN antibody, anti-RNU2AF1,35-KD subunit antibody, anti-U2 auxiliary factor 35 kDa subunit antibody, anti-U2 small nuclear RNA auxiliary factor 1 antibody, anti-U2 small nuclear RNA auxillary factor 1 antibody, anti-U2 snRNP auxiliary factor small subunit antibody
7 Results for "u2af35" in Primary Antibodies
![Western Blot: U2AF35 Antibody [NBP1-32515] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Western-Blot-NBP1-32515-img0006.jpg "Western Blot: U2AF35 Antibody [NBP1-32515] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-32515] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32515-img0004.jpg)
![Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515] - Paraffin-embedded mouse intestine. U2AF35 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunohistochemistry-Paraffin-NBP1-32515-img0007.jpg)
![Immunoprecipitation: U2AF35 Antibody [NBP1-32515] - U2AF35 protein from 293T whole cell extracts using 5 ug of U2AF35 antibody. Western blot analysis was performed using U2AF35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunoprecipitation-NBP1-32515-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: U2AF35 Antibody [NBP1-32515] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with U2AF35 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32515_rabbit-polyclonal-u2af35-antibody-1610202419414536.jpg)
![Western Blot: U2AF35 Antibody [NBP1-92553] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Western-Blot-NBP1-92553-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: U2AF35 Antibody [NBP1-92553] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-92553] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92553-img0005.jpg)
![Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunohistochemistry-Paraffin-NBP1-92553-img0004.jpg)
![Western Blot: U2AF35 Antibody [NBP1-19120] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. U2AF35 was also immunoprecipitated by rabbit anti-U2AF35 antibody NBP1-19121.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Western-Blot-NBP1-19120-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: U2AF35 Antibody [NBP1-19120] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. U2AF35 was also immunoprecipitated by rabbit anti-U2AF35 antibody NBP1-19121.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-19120] - Section of human lung carcinoma (left) and mouse CT26 colon carcinoma (right).](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunohistochemistry-Paraffin-NBP1-19120-img0004.jpg)
![Immunohistochemistry: U2AF35 Antibody [NBP3-35321] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using U2AF35 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35321_rabbit-u2af35-pab-1012202411381238.jpg "Immunohistochemistry: U2AF35 Antibody [NBP3-35321] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using U2AF35 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunoprecipitation: U2AF35 Antibody [NBP3-35321] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug U2AF35 antibody. Western blot was performed from the immunoprecipitate using U2AF35 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35321_rabbit-u2af35-pab-1012202411334072.jpg)
![Western Blot: U2AF35 Antibody [NBP3-35321] - Western blot analysis of various lysates using U2AF35 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35321_rabbit-u2af35-pab-1012202411381253.jpg)
![Immunohistochemistry: U2AF35 Antibody (1E9) [NBP3-43059] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (U2AF35 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-43059_mouse-u2af35-mab-1e9-101220241723640.jpg "Immunohistochemistry: U2AF35 Antibody (1E9) [NBP3-43059] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (U2AF35 Antibody) at dilution of 1:50")
![Immunoprecipitation: U2AF35 Antibody (1E9) [NBP3-43059] - IP Result of anti-U2AF35 (IP:FNab09138, 3ug; Detection:FNab09138 1:500) with HeLa cells lysate 3000ug.](http://images.novusbio.com/fullsize/nbp3-43059_mouse-u2af35-mab-1e9-101220241715478.jpg)
![Western Blot: U2AF35 Antibody (1E9) [NBP3-43059] - HeLa cells were subjected to SDS PAGE followed by western blot with (U2AF35 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-43059_mouse-u2af35-mab-1e9-10122024172366.jpg)
![Immunohistochemistry: U2AF35 Antibody [NBP2-46797] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunohistochemistry-NBP2-46797-img0001.jpg "Immunohistochemistry: U2AF35 Antibody [NBP2-46797] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.")
![Sandwich ELISA: U2AF35 Antibody (1C8) [H00007307-M01] - Detection limit for recombinant GST tagged U2AF1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/U2AF35-Antibody-1C8-Sandwich-ELISA-H00007307-M01-img0001.jpg "Sandwich ELISA: U2AF35 Antibody (1C8) [H00007307-M01] - Detection limit for recombinant GST tagged U2AF1 is 0.1 ng/ml as a capture antibody.")