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TXNL1 Antibody

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Western Blot: TXNL1 Antibody [NBP1-86899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: TXNL1 Antibody [NBP1-86899] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TXNL1 Antibody [NBP1-86899] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Western Blot: TXNL1 Antibody [NBP1-86899] - Analysis in human cell line HL-60.
Immunohistochemistry-Paraffin: TXNL1 Antibody [NBP1-86899] - Staining of mouse hypothalamus shows positivity in the neurons of paraventricular nucleus.
Immunohistochemistry-Paraffin: TXNL1 Antibody [NBP1-86899] - Staining of mouse facial nucleus shows positivity in neurons.
Immunohistochemistry-Paraffin: TXNL1 Antibody [NBP1-86899] - Staining of human cerebral cortex shows positivity in subsets of neurons.
Immunohistochemistry-Paraffin: TXNL1 Antibody [NBP1-86899] - Staining of human hippocampus shows moderate nuclear and cytoplasmic immunoreactivity in neurons.
Immunohistochemistry-Paraffin: TXNL1 Antibody [NBP1-86899] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: TXNL1 Antibody [NBP1-86899] - Immunofluorescence staining of mouse dentate gyrus shows positivity in a subset of neurons in the polymorph layer .
Immunohistochemistry-Paraffin: TXNL1 Antibody [NBP1-86899] - Immunofluorescence staining of mouse brain shows positivity in neuronal cells in the motor cortex.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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TXNL1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CLNESDEHGFDNCLRKDTTFLESDCDEQLLITVAFNQPVKLYSMKFQGPDNGQGPKYVKIFINLPRSMDFEEAERSEPTQALELTEDDIKEDGIVPLRYVKFQNVNSVTIFVQSNQGEEETTRISYFTFIGTPVQATNMNDFKRVVGK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TXNL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TXNL1 Protein (NBP1-86899PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TXNL1 Antibody

  • 32 kDa thioredoxin-related protein
  • thioredoxin-like 1
  • thioredoxin-like, 32kDa
  • thioredoxin-related 32 kDa protein
  • thioredoxin-related protein 1
  • TRP32thioredoxin-like protein 1
  • Txl
  • TXL-1
  • TXNL

Background

Thioredoxins are a group of small redox active proteins which have a conserved active site sequence. The predicted 32.3-kD protein of TXNL1 (Thioredoxin like protein 1) has 2 distinct domains: an N terminal domain, which is 43% identical to human TXN, and a C terminal domain, which showed no homology to other proteins in the sequence databases. The active site sequence, located within the N terminal domain, is that of a thioredoxin like protein; compared to the active site sequence of TXN, it has a single amino acid substitution. Unlike other thioredoxin like proteins, TXNL1 did not act as a substrate for thioredoxin reductase in an insulin assay.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TXNL1 Antibody (NBP1-86899) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TXNL1