![Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20760-img0001.jpg "Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:10000.")
| Reactivity | Hu, Dr, Ze, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary. |
| Marker | Microtubule Marker |
| Predicted Species | Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TUBA1B |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Tubulin alpha-1B Antibody (NBP2-20760)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TUBA1B |
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20760] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBA1B protein stained by alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20760-img0003.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20760] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBA1B protein stained by alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20760] - Human colon carcinoma, using alpha Tubulin antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-20760-img0002.jpg "Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20760] - Human colon carcinoma, using alpha Tubulin antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20760] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Tubulin alpha-1B antibody (NBP2-20760) dilution: 1:200.](http://images.novusbio.com/fullsize/nbp2-20760_rabbit-polyclonal-tubulin-alpha-1b-antibody-91020241957217.jpg "Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20760] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Tubulin alpha-1B antibody (NBP2-20760) dilution: 1:200.")
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20760) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20760_rabbit-polyclonal-tubulin-alpha-1b-antibody-91020242001045.jpg "Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20760) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")