![Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser150-Antibody-Western-Blot-NBP2-29526-img0002.jpg "Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.")
| Reactivity | Hu, Mu, Rt, Hu, PmSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 conjugated to KLH. Accession # P48787 |
| Modification | p Ser150 |
| Specificity | Specific for endogenous levels of the ~25 kDa Troponin I Type 3 (cardiac) protein phosphorylated at Ser150. Immunolabeling is greatly decreased with lambda-phosphatase treatment. |
| Preparation Method |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. |
| Predicted Species | Human (100%), Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TNNI3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
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| Publication using NBP2-29526 | Applications | Species |
|---|---|---|
| Zhu N, Yi B, Guo Z et al. Pim-1 Kinase Phosphorylates Cardiac Troponin I and Regulates Cardiac Myofilament Function. Cell. Physiol. Biochem. 2018-03-10 [PMID: 29544221] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Troponin I Type 3 (cardiac) Antibody (NBP2-29526)Find related products by research area.
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Troponin I Type 3 (TnI, cardiac) Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components... Read full blog post. |
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![Immunofluorescence: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Fixed adult mouse cardiomyocyte were stained with anti-Pim-1 and anti-cTnI antibodies and then processed for fluorescentstaining analysis. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser150-Antibody-Immunofluorescence-NBP2-29526-img0003.jpg "Immunofluorescence: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Fixed adult mouse cardiomyocyte were stained with anti-Pim-1 and anti-cTnI antibodies and then processed for fluorescentstaining analysis. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.")
![Immunoprecipitation: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Adult mouse heart tissue samples were extracted and immunoprecipitated with either anti-Pim-1 antibody or control IgG and then analyzed by Westernblot. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser150-Antibody-Immunoprecipitation-NBP2-29526-img0004.jpg "Immunoprecipitation: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Adult mouse heart tissue samples were extracted and immunoprecipitated with either anti-Pim-1 antibody or control IgG and then analyzed by Westernblot. From Zhu et al.: Phosphorylation of cTnI by Pim1 Kinase, Cell Physiol Biochem 2018;45:2174-2186.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")