Novus Biologicals products are now on bio-techne.com

Troponin I type 2 (fast skeletal) Antibody (OTI3A11)

Images

 
Western Blot: Troponin I type 2 (fast skeletal) Antibody (3A11) [NBP2-46171] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Troponin I type 2.
Immunohistochemistry: Troponin I type 2 (fast skeletal) Antibody (3A11) [NBP2-46171] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: Troponin I type 2 (fast skeletal) Antibody (3A11) [NBP2-46171] - Analysis of extracts (10ug) from HepG2 cell line.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI3A11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Troponin I type 2 (fast skeletal) Antibody (OTI3A11) Summary

Immunogen
Full length human recombinant protein of human TNNI2 (NP_003273) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TNNI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Troponin I type 2 (fast skeletal) Antibody (OTI3A11)

  • skeletal, fast
  • troponin I type 2 (skeletal, fast)
  • troponin I, fast skeletal muscle
  • Troponin I, fast-twitch isoform
  • troponin I, fast-twitch skeletal muscle isoform

Background

The troponin I type 2 gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-95197
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-26199
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP1-56641
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-35336
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-89707
Species: Hu
Applications: IHC, IHC-P
NBP2-41309
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-37958
Species: Hu
Applications: IHC, IHC-P
NBP2-93498
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB120-11099
Species: Al, Am, Av, Bv, Ch, Fi, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, WB
NBP2-16818
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, WB
NBP1-33716
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89707
Species: Hu
Applications: IHC, IHC-P
H00007138-B01P
Species: Hu, In
Applications: WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NB110-68136
Species: Fe, Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-83077
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB

Publications for Troponin I type 2 (fast skeletal) Antibody (NBP2-46171) (0)

There are no publications for Troponin I type 2 (fast skeletal) Antibody (NBP2-46171).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Troponin I type 2 (fast skeletal) Antibody (NBP2-46171) (0)

There are no reviews for Troponin I type 2 (fast skeletal) Antibody (NBP2-46171). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Troponin I type 2 (fast skeletal) Antibody (NBP2-46171) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Troponin I type 2 (fast skeletal) Products

Research Areas for Troponin I type 2 (fast skeletal) Antibody (NBP2-46171)

Find related products by research area.

Blogs on Troponin I type 2 (fast skeletal).

Troponin I Type 2 - I stay with fast-twitch skeletal muscles only
The protein Troponin I is a component of the heteromeric protein complex responsible for regulating both skeletal and cardiac muscle contraction. Troponin complex is made up of three parts: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Troponin I type 2 (fast skeletal) Antibody (OTI3A11) and receive a gift card or discount.

Bioinformatics

Gene Symbol TNNI2