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Troponin I type 2 (fast skeletal) Antibody (2D5)

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Western Blot: Troponin I type 2 (fast skeletal) Antibody (2D5) [H00007136-M05] - Analysis of TNNI2 expression in transfected 293T cell line by TNNI2 monoclonal antibody (M05), clone 2D5. Lane 1: TNNI2 transfected lysatE ...read more
Sandwich ELISA: Troponin I type 2 (fast skeletal) Antibody (2D5) [H00007136-M05] - Detection limit for recombinant GST tagged TNNI2 is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Troponin I type 2 (fast skeletal) Antibody (2D5) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
TNNI2 (NP_003273.1, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDEEKRNRAITARRQHLKSVMLQIAATELEKEESRREAEKQNYLAEHCPPLHIPGSMSEVQELCKQLHAKIDAAEEEKYDMEVRVQKTSKELEDMNQKL
Specificity
Reacts with troponin I type 2 (skeletal, fast).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNNI2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Troponin I type 2 (fast skeletal) Antibody (2D5)

  • skeletal, fast
  • troponin I type 2 (skeletal, fast)
  • troponin I, fast skeletal muscle
  • Troponin I, fast-twitch isoform
  • troponin I, fast-twitch skeletal muscle isoform

Background

This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Research Areas for Troponin I type 2 (fast skeletal) Antibody (H00007136-M05)

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Blogs on Troponin I type 2 (fast skeletal).

Troponin I Type 2 - I stay with fast-twitch skeletal muscles only
The protein Troponin I is a component of the heteromeric protein complex responsible for regulating both skeletal and cardiac muscle contraction. Troponin complex is made up of three parts: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI2
Entrez
Uniprot