![Immunohistochemistry: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Immunofluorescence analysis of paraffin-embedded human striated muscle tissue labeling tropomyosin-2 with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33059, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33059_rabbit-tropomyosin-2-mab-psh02-63-298202414262682.jpg "Immunohistochemistry: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Immunofluorescence analysis of paraffin-embedded human striated muscle tissue labeling tropomyosin-2 with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33059, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | PSH02-63 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within human tropomyosin-2 aa 200-250 / 284. (Uniprot: P07951) |
| Localization | Cytoplasm, cytoskeleton. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TPM2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | TPM2 |
![Immunohistochemistry: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33059) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33059_rabbit-tropomyosin-2-mab-psh02-63-29820241435247.jpg "Immunohistochemistry: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33059) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Western blot analysis of tropomyosin-2 on different lysates with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: Saos-2 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: Mouse skeletal muscle tissue lysate Lane 6: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 37 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33059_rabbit-tropomyosin-2-mab-psh02-63-392024848500.jpg "Western Blot: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Western blot analysis of tropomyosin-2 on different lysates with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: Saos-2 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: Mouse skeletal muscle tissue lysate Lane 6: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 37 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")