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TRF-2 Antibody

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human TRF2 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

TRF-2 Antibody Summary

Immunogen
E. coli-derived recombinant human TRF2
Gln78-Thr238
Accession # Q15554
Specificity
Detects endogenous human TRF2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TERF2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRF-2 Antibody

  • Telomeric DNA-binding protein
  • telomeric repeat binding factor 2
  • Telomeric repeat binding protein 2
  • telomeric repeat-binding factor 2
  • TERF2
  • TRBF2
  • TRF2
  • TRF-2
  • TTAGGG repeat binding factor 2
  • TTAGGG repeat-binding factor 2

Background

TRF2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55‑60 kDa, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF1 to promote normal telomere length. TRF2 has been found up regulated in several human cancers. Human TRF2 is 500 amino acids (aa) in length. It contains an N-terminal Arg-rich region (aa 13‑30), a dimerization domain (aa 46‑112), an NLS (aa 329‑333), and a DNA binding HTH myb-type domain (aa 442‑499). There is one potential alternate start site 42 aa upstream of the standard start site, and one splice form that shows a 13 aa substitution for aa 239‑500. Over aa 78‑238, human TRF2 shares 96% aa identity with mouse TRF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TRF-2 Antibody (AF5635). (Showing 1 - 5 of 5 FAQ).

  1. Are the TRF-2 antibodies validated in Simple Western?
    • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
  2. Does TRF-2 antibodies comes in lyophilized form?
    • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
  3. What the theoretical molecular weight for TRF-2 antibodies?
    • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.
  4. Does this TRF-2 antibody come in lyophilized form?
    • Yes, we carry this TRF-2 antibodies in lyophilized format.
  5. What is the immunogen sequence of this TRF-2 antibody?
    • A sequence from Gln78 - Thr238.

Secondary Antibodies

 

Isotype Controls

Additional TRF-2 Products

Blogs on TRF-2.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

TRF2: Telomere Maintenance and Protection
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an...  Read full blog post.

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Bioinformatics

Gene Symbol TERF2
Uniprot