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Trappin-2/Elafin/Skalp Antibody

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Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) activity is measured in the presence of Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747‑PI) that has been pre­incubated with increasing concentrations of Goat Anti-Human Trappin‑2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747). The ND50 is typically 15.6 µg/mL.
Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) activity is measured in the presence of Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747‑PI) that has been pre­incubated with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1747
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Catalog# & Formulation Size Price
Carrier Free
AF1747-SP
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Trappin-2/Elafin/Skalp Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trappin‑2/Elafin
Ala23-Gln117
Accession # P19957
Specificity
Detects human Trappin‑2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM-2, and human Neutrophil Elastase is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PI3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation 25 ug/mL
  • Neutralization 15.6 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human Trappin-2/Elafin Antibody (Catalog # AF1747)
ELISA Detection: Human Trappin-2/Elafin Biotinylated Antibody (Catalog # BAF1747)
Standard: Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI)
Publications
Read Publications using
AF1747 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trappin-2/Elafin/Skalp Antibody

  • cementoin
  • Elafin
  • Elastase-specific inhibitor
  • ESI
  • ESIWAP four-disulfide core domain protein 14
  • Peptidase inhibitor 3
  • peptidase inhibitor 3, skin-derived
  • PI3
  • PI-3
  • pre-elafin
  • protease inhibitor 3, skin-derived (SKALP)
  • Protease inhibitor WAP3
  • SKALP
  • SKALPELAFIN
  • Skin-derived antileukoproteinase
  • Trappin2
  • Trappin-2
  • WAP four-disulfide core domain 14
  • WAP3MGC13613
  • WFDC14elafin

Background

Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full‑length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.

  1. Schalkwijk, J. et al. (1999) Biochem. J. 340:569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Trappin-2/Elafin/Skalp
AF1747
Trappin-2/Elafin/Skalp Antibody
Species: Hu
Applications: WB, IP, B/N, ELISA(Cap)

Publications for Trappin-2/Elafin/Skalp Antibody (AF1747)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Bioassay, ELISA Detection, ELISA Development, IHC-Fr, Immunodepletion.


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(1)
ELISA Detection
(1)
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(1)
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(1)
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Human
(5)
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Showing Publications 1 - 7 of 7.
Publications using AF1747 Applications Species
J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants Bone Rep, 2020-09-11;13(0):100715. 2020-09-11 [PMID: 32995386] (ELISA Detection, Human) ELISA Detection Human
Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 8/1/2020 [PMID: 32645325]
J Wang, C Ortiz, L Fontenot, Y Xie, W Ho, SA Mattai, DQ Shih, HW Koon High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures PLoS ONE, 2020-04-14;15(4):e0231796. 2020-04-14 [PMID: 32287314] (Bioassay, Human) Bioassay Human
Burgener , Adam, Tjernlund , Annelie, Kaldensjo , Tove, Abou , Max, McCorrister , Stuart, Westmacott , Garrett, Mogk , Kenzie, Ambrose , Emma, Broliden , Kristina, Ball , Blake A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol, 2013-02-28;87(9):5141-50. 2013-02-28 [PMID: 23449785] (IHC-Fr, Human) IHC-Fr Human
Anna G. Drannik, Kakon Nag, Xiao-Dan Yao, Bethany M. Henrick, T. Blake Ball, Francis A. Plummer et al. Anti-HIV-1 Activity of Elafin Depends on Its Nuclear Localization and Altered Innate Immune Activation in Female Genital Epithelial Cells PLoS ONE 12/27/2012 [PMID: 23300756]
Drannik AG, Nag K, Yao XD, Henrick BM, Jain S, Ball TB, Plummer FA, Wachihi C, Kimani J, Rosenthal KL Anti-HIV-1 activity of elafin is more potent than its precursor's, trappin-2, in genital epithelial cells. J. Virol., 2012-02-15;86(8):4599-610. 2012-02-15 [PMID: 22345469] (Immunodepletion, Human) Immunodepletion Human
Guyot N, Butler MW, McNally P, Weldon S, Greene CM, Levine RL, O'Neill SJ, Taggart CC, McElvaney NG Elafin, an Elastase-specific Inhibitor, Is Cleaved by Its Cognate Enzyme Neutrophil Elastase in Sputum from Individuals with Cystic Fibrosis. J. Biol. Chem., 2008-09-17;283(47):32377-85. 2008-09-17 [PMID: 18799464] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol PI3
Uniprot