Immunocytochemistry/ Immunofluorescence reported in scientific literature (Zahoor et al (2010))
Immunoprecipitation 1:10-1:500. Use reported in scientific literature (Zahoor et al (2010))
Western Blot 1:100-1:2000. Use reported in scientific literature (Zahoor et al (2010))
Application Notes
Confocal immunofluorescence: Cells were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.5 % Triton X-100 for 20 min at room temperature (RT). The cells were blocked with PBS containing 10 % BSA and probed with NIBP pAB for 1 hour at RT. After washing with PBS, cells were incubated with Alexa conjugated goat anti-mouse antibodies. See Zahoor et al for details (2010).
Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in human (isoform CRA_A and CRA_b), chimpanzee, monkey, mouse, opossum, rat and dog; xenopus (94%).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole Rabbit antisera with 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified
Alternate Names for TRAPPC9 Antibody
IKBKBBP
IKK2 binding protein
KIAA1882IBP
MRT13MGC4769
NIBPMGC4737
NIK- and IKBKB-binding protein
NIK and IKK-beta binding protein
T1
trafficking protein particle complex 9
trafficking protein particle complex subunit 9
TRAPP 120 kDa subunit
TRS120
Tularik gene 1 protein
Background
NIBP is a 1246 amino acid intracellular regulatory protein. Reports suggest that NIBP physically interacts with NIK, IKK (beta), but not IKK (alpha) or IKK (gamma) and thus acts as a novel enhancer of cytokine-induced NF-(kappa) B signaling pathway. Due to its neuronal expression NIBP is considered as a potential target for modulating the NF-(kappa) B signaling cascade in neuronal pathologies dependent upon abnormal activation of this pathway. NIBP is mainly expressed in brain, muscle, heart, and kidney.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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