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Transglutaminase 2/TGM2 Antibody

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Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Analysis of extracts from K562 cells using Transglutaminase 2 antibody at 1:100.
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of Transglutaminase 2/TGM2 antibody (NBP2-24470). The staining was ...read more
Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Analysis of Transglutaminase 2 in A) BxPC-3 cell lysate using NBP2-24470 at 2.0 ug/ml and B) MCF-7 negative control cell lysate.
Simple Western: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Simple Western lane view shows a specific band for Transglutaminase 2/TGM2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Pm, PmSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Transglutaminase 2/TGM2 Antibody Summary

Immunogen
A portion of amino acids 600-650 of human Transglutaminase 2 was used as the immunogen.
Predicted Species
Chimpanzee (93%), Monkey (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 1:50
  • Western Blot 2-5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Publications
Read Publication using NBP2-24470.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Transglutaminase 2/TGM2 Antibody

  • C polypeptide
  • EC 2.3.2.13
  • G-ALPHA-h
  • protein-glutamine gamma-glutamyltransferase 2
  • protein-glutamine-gamma-glutamyltransferase
  • TG(C)
  • TG2
  • TGase C
  • TGase H
  • TGase-2
  • TGase-H
  • TGC
  • TGCGNAH
  • TGM2
  • Tissue transglutaminase
  • transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
  • Transglutaminase 2
  • Transglutaminase C
  • Transglutaminase H
  • transglutaminase-2
  • tTG

Background

Transglutaminase 2 is historically a member of the transglutaminase family that catalyzes a calcium-dependent acyl transfer reaction between peptide bound glutamine and a suitable primary amine.The protein is known to participate in all-trans-retinoic acid mediated signaling events as a G-protein and transamidate targets such as RhoA and the retinoblastoma gene product. The action of transglutaminase 2 stabilizes proteins by conferring mechanical resistance and protection from proteolysis. The protein is expressed intracellularly and secreted on the cell surface where it interacts with proteins in the extracellular matrix and aids in anchoring cells to basement membranes. Recently, transglutaminase 2 has been identified as an important factor that interacts with fibronectin in the extracellular matrix at the cell membrane and provides survival signals and inhibits anoikis in fibroblasts. The protein is expressed in human endothelial cells. The human transglutaminase 2 gene is localized in the chromosomal region 20q12. Transcript variants encoding two different isoforms have been found for this gene. Deficiency of the protein results in early-onset diabetes type 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Transglutaminase 2/TGM2 Antibody (NBP2-24470)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TGM2
Uniprot