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Transferrin Antibody (HTF-14) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Po, Rb, Bv(-), Ca(-), Eq(-), Sh(-)Species Glossary
Applications WB, ELISA, Func, ICC/IF, IHC, B/N
Clone
HTF-14
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

Transferrin Antibody (HTF-14) [Janelia Fluor® 646] Summary

Immunogen
Purified porcine transferrin.
Specificity
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TF
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Radioimmunoassay
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Ion exchange chromatography

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Transferrin Antibody (HTF-14) [Janelia Fluor® 646]

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Po, Rb, Bv(-), Ca(-), Eq(-), Sh(-)
Applications: WB, ELISA, Func, ICC/IF, IHC, B/N

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Transferrin Antibody (NB500-418JF646)

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol TF