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TRADD Antibody [Unconjugated]

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRADD at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRADD ...read more
TRADD was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human TRADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2658) at 1.7 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2658-SP
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TRADD Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TRADD
Met1-Ala312
Accession # Q15628
Specificity
Detects human TRADD.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRADD
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1-25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRADD Antibody [Unconjugated]

  • MGC11078
  • TNFRSF1A-associated via death domainTNFR1-associated DEATH domain protein
  • TRADD
  • tumor necrosis factor receptor type 1 associated death domain protein
  • tumor necrosis factor receptor type 1-associated DEATH domain protein
  • tumor necrosis factor receptor-1-associated protein

Background

TRADD (TNF R1A-associated death domain protein) is a 34 kDa protein that is expressed nearly ubiquitously. TRADD functions as an adaptor protein associating with the cytoplasmic domain of TNF receptor 1 or to another adaptor, FADD (Fas-interacting DD) to form the DISC (death inducing signaling complex). Human TRADD is an alpha-helical, Greek key, death domain-containing protein that is 77% identical to both mouse and rat TRADD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TRADD
Uniprot