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TPD52L1/D53 Antibody

Images

 
Western Blot: TPD52L1/D53 Antibody [NBP1-84313] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line MCF-7
Immunocytochemistry/ Immunofluorescence: TPD52L1/D53 Antibody [NBP1-84313] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and cell junctions.
Orthogonal Strategies: Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313] - Staining in human salivary gland and lymph node tissues using anti-TPD52L1 antibody. Corresponding TPD52L1 RNA-seq data ...read more
Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313] - Staining of human lymph node shows low expression as expected.
Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313] - Staining of human salivary gland shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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TPD52L1/D53 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MEAQAQGLLETEPLQGTDEDAVASADFSSMLSEEEKEELKAELVQLEDEITTLRQVLSAKERHLVEI
Predicted Species
Mouse (94%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPD52L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TPD52L1/D53 Protein (NBP1-84313PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TPD52L1/D53 Antibody

  • D53
  • MGC8556
  • tumor protein D52-like 1hD53TPD52L2
  • tumor protein D52-like 2
  • tumor protein D53

Background

TPD52L1/D53 encodes a member of the tumor protein D52 (TPD52) family. The encoded protein contains a coiled-coil domain and may form homo- or hetero-dimer with TPD52 family members. The protein is reported to be involved in cell proliferation and calcium signaling. It also interacts with the mitogen-activated protein kinase kinase kinase 5 (MAP3K5/ASK1) and positively regulates MAP3K5-induced apoptosis. Multiple alternatively spliced transcript variants have been observed, but the full-length nature of some variants has not yet been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TPD52L1