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TORC2 Overexpression Lysate

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Western Blot: Torc2 Overexpression Lysate (Adult Normal) [NBL1-09492] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Torc2.
Western Blot: Torc2 Overexpression Lysate (Adult Normal) [NBL1-09492] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Torc2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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TORC2 Overexpression Lysate Summary

Description

TORC2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208836

Accession#: NM_181715

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CRTC2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
73.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for TORC2 Overexpression Lysate

  • CREB regulated transcription coactivator 2
  • CREB-regulated transcription coactivator 2
  • CRTC2
  • TORC2
  • TORC-2
  • TORC2transducer of regulated cAMP response element-binding protein (CREB) 2
  • Transducer of CREB protein 2
  • Transducer of regulated cAMP response element-binding protein 2

Background

TORC2 (transducer of regulated CREB activity 2) is part of a family of CREB (cAMP response element-binding protein) coactivators that also includes TORC1 and TORC3. These proteins function to activate CREB-dependent transcription in response to cAMP and calcium signaling pathways. TORC2 has been found to be pivotal to the process of gluconeogenesis. TORC2 is also known as CREB regulated transcription activator 2, and CRTC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CRTC2