TOPORS Antibody Summary
Immunogen |
The immunogen recognized by this antibody maps to a region between residue 995 and 1045 of human topoisomerase I binding, arginine/serine-rich using the numbering given in entry topoisomerase I binding, arginine/serine-rich NP_005793.2 (GeneID 10210). |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
TOPORS |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 1:100 - 1:500
- Immunohistochemistry-Paraffin 1:100 - 1:500
- Immunoprecipitation 5-15 ug/mg lysate
- Western Blot 1:2000-1:10000
|
Application Notes |
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
TBS and 0.1% BSA |
Preservative |
0.09% Sodium Azide |
Concentration |
0.2 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for TOPORS Antibody
Background
TOPORS (topoisomerase I-binding RS protein) was identified in a a yeast two-hybrid screen for proteins that bind the N-terminus of topoisomerase I. TOPORS contains a RING-type zinc-finger domain and an RS-rich region. TOPORS has been found to possess both ubiquitin and SUMO-1 E3 ligase activity. It can ubiquitinate and sumoylate p53 and may possibly function as a tumor suppressor. Defects in TOPORS are the cause of retinitis pigmentosa type 31.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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