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TOPORS Antibody

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Independent Antibodies: Western Blot: TOPORS Antibody [NBP1-22976] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-TOPORS antibody NBP1-22976. TOPORS was also immunoprecipitated by another ...read more
Immunohistochemistry-Paraffin: TOPORS Antibody [NBP1-22976] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TOPORS used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

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TOPORS Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 995 and 1045 of human topoisomerase I binding, arginine/serine-rich using the numbering given in entry topoisomerase I binding, arginine/serine-rich NP_005793.2 (GeneID 10210).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TOPORS
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TOPORS Antibody

  • EC 6.3.2.-
  • LUNtopoisomerase I binding, arginine/serine-rich
  • p53-binding protein 3
  • p53BP3
  • retinitis pigmentosa 31 (autosomal dominant)
  • RP31
  • SUMO1-protein E3 ligase Topors
  • topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase
  • Topoisomerase I-binding arginine/serine-rich protein
  • Topoisomerase I-binding RING finger protein
  • TP53BPLE3 ubiquitin-protein ligase Topors
  • Tumor suppressor p53-binding protein 3

Background

TOPORS (topoisomerase I-binding RS protein) was identified in a a yeast two-hybrid screen for proteins that bind the N-terminus of topoisomerase I. TOPORS contains a RING-type zinc-finger domain and an RS-rich region. TOPORS has been found to possess both ubiquitin and SUMO-1 E3 ligase activity. It can ubiquitinate and sumoylate p53 and may possibly function as a tumor suppressor. Defects in TOPORS are the cause of retinitis pigmentosa type 31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TOPORS
Entrez
Uniprot