![Western Blot: TOP2B Antibody [NB100-40842] - Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence withan exposure time of 30 seconds](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NB100-40842-img0010.jpg "Western Blot: TOP2B Antibody [NB100-40842] - Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence withan exposure time of 30 seconds")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1570 and the C-terminus (residue 1621) of human DNA Topoisomerase II Beta using the numbering given in entry NP_001059.2 (GeneID 7155). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TOP2B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Theoretical MW | 183 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||
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reviewed by:
Verified Customer |
WB | Human | 08/24/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for TOP2B Antibody (NB100-40842)Find related products by research area.
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![Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbitanti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NB100-40842-img0009.jpg "Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbitanti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB")
![Western Blot: TOP2B Antibody [NB100-40842] - Detection of Human Topo II Beta on HeLa whole cell lysate using NB100-40842. Topo II Beta was also immunoprecipitated by another rabbit anti-Topo II Beta antibody and NB100-40841.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NB100-40842-img0007.jpg "Western Blot: TOP2B Antibody [NB100-40842] - Detection of Human Topo II Beta on HeLa whole cell lysate using NB100-40842. Topo II Beta was also immunoprecipitated by another rabbit anti-Topo II Beta antibody and NB100-40841.")
![Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NB100-40842-img0008.jpg "Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
