TOP2B Antibodies
We offer TOP2B Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each TOP2B Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TOP2B Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the TOP2B Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TOP2B polyclonal antibodies and browse our TOP2B monoclonal antibody catalog.
Alternate Names for TOP2B Antibodies
anti-TOP2B antibody, anti-antigen MLAA-44 antibody, anti-DNA topoisomerase 2-beta antibody, anti-DNA topoisomerase II beta antibody, anti-DNA topoisomerase II, 180 kD antibody, anti-DNA topoisomerase II, beta isozyme antibody, anti-EC 5.99.1.3 antibody, anti-top2beta antibody, anti-TOPIIB antibody, anti-topo II beta antibody, anti-topoisomerase (DNA) II beta (180kD) antibody, anti-topoisomerase (DNA) II beta 180kDa antibody, anti-topoisomerase II beta antibody, anti-topoisomerase IIb antibody, anti-U937 associated antigen antibody
9 Results for "top2b" in Primary Antibodies
![Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NBP1-89527-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP1-89527] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89527-img0011.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human pancreas shows weak to moderate nuclear positivity in islets of Langerhans and exocrine glandular cells.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89527-img0020.jpg)
![Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NBP1-89527-img0012.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89527-img0017.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89527-img0018.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human kidney shows moderate nuclear positivity in cells in glomeruli and tubules.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89527-img0019.jpg)
![Simple Western: TOP2B Antibody [NBP1-89527] - Simple Western lane view shows a specific band for TOP2B in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Simple-Western-NBP1-89527-img0007.jpg)
![Simple Western: TOP2B Antibody [NBP1-89527] - Electropherogram image(s) of corresponding Simple Western lane view. TOP2B antibody was used at 1:100 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/TOP2B-Antibody-Simple-Western-NBP1-89527-img0008.jpg)
![Western Blot: TOP2B Antibody [NB100-40842] - Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence withan exposure time of 30 seconds](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NB100-40842-img0010.jpg "Western Blot: TOP2B Antibody [NB100-40842] - Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence withan exposure time of 30 seconds")
![Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbitanti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NB100-40842-img0009.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TOP2B Antibody [NB100-40842] - Detection of Human Topo II Beta on HeLa whole cell lysate using NB100-40842. Topo II Beta was also immunoprecipitated by another rabbit anti-Topo II Beta antibody and NB100-40841.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NB100-40842-img0007.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NB100-40842-img0008.jpg)
![Western Blot: TOP2B Antibody [NBP2-20684] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NBP2-20684-img0007.jpg "Western Blot: TOP2B Antibody [NBP2-20684] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP2-20684] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20684-img0012.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse brain. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP2-20684-img0011.jpg)
![Western Blot: TOP2B Antibody [NBP2-20684] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/Topo-II-beta-Antibody-Western-Blot-NBP2-20684-img0001.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Ovarian cancer, using Topoisomerase II beta antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Topo-II-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-20684-img0002.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse brain. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP2-20684-img0010.jpg)
![Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse testis. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunohistochemistry-Paraffin-NBP2-20684-img0009.jpg)
![Immunoprecipitation: TOP2B Antibody [NBP2-20684] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 ug of Topoisomerase II beta antibody 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunoprecipitation-NBP2-20684-img0003.jpg)
![Western Blot: TOP2B Antibody [NBP2-58728] - Analysis using Anti-TOP2B antibody NBP2-58728 (A) shows similar pattern to independent antibody NBP1-89527 (B).](http://images.novusbio.com/fullsize/TOP2B-Antibody-Western-Blot-NBP2-58728-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TOP2B Antibody [NBP2-58728] - Analysis using Anti-TOP2B antibody NBP2-58728 (A) shows similar pattern to independent antibody NBP1-89527 (B).")
![Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP2-97789] - Immunofluorescence staining of TOP2B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TOP2B polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.](http://images.novusbio.com/fullsize/TOP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97789-img0001.jpg "Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP2-97789] - Immunofluorescence staining of TOP2B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TOP2B polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.")